Clean hands and vial. Draw 1-2mL diluent, inject gently into HGH vial. Swirl to mix (don’t shake). Store at 2-8°C. Use as directed, dispose safely.
RECONSTITUTE HGH USING FOR IVF TREATMENT hgh injection
Mixing Instructions for HGH (Human Growth Hormone)
Proper mixing (reconstitution) of HGH is critical to ensure its effectiveness and minimize risks of degradation or contamination. Always follow the manufacturer’s guidelines, but here is a general protocol:
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Materials Needed
1. **Lyophilized HGH vial** (powder form).
2. **Diluent** (as recommended by the manufacturer):
- **Bacteriostatic Water** (preferred for longer shelf life).
- **Sterile Water for Injection** (use within a shorter period).
3. Alcohol swabs.
4. Sterile syringe and needle (typically 1 mL or 3 mL).
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Preparation Steps:
1. **Sanitize Hands and Work Area:**
- Wash hands thoroughly with soap and water or use a hand sanitizer.
- Clean your work surface with alcohol wipes to ensure sterility.
2. **Prepare the Vial and Diluent:**
- Remove the caps from the HGH vial and the diluent vial (if applicable).
- Wipe the rubber stoppers on both vials with an alcohol swab and let them dry.
3. **Draw the Diluent into the Syringe:**
- Attach a sterile needle to the syringe.
- Insert the needle into the diluent vial and draw the required volume of diluent.
- Common volume: **1–2 mL**, but refer to the manufacturer's instructions for the exact amount.
- Remove air bubbles by gently tapping the syringe and expelling any air.
4. **Reconstitute the HGH:**
- Insert the needle into the HGH vial at an angle to avoid directly hitting the powder.
- Slowly and gently inject the diluent down the side of the vial to minimize foaming.
- Do not inject the diluent forcefully, as this can damage the fragile HGH molecules.
5. **Mix the Solution:**
- Gently swirl or roll the vial between your fingers until the powder is fully dissolved.
- **Do not shake the vial**, as this can denature the HGH.
- Inspect the solution: it should be clear and free of particles.
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Storage After Mixing:
- Place the reconstituted HGH vial in the refrigerator immediately (2°C to 8°C).
- **Bacteriostatic Water:** Stable for up to **14–28 days**.
- **Sterile Water:** Use within **24–48 hours**.
- Avoid freezing the solution, as this will degrade the HGH.
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Precautions:
- Always use sterile equipment and follow aseptic techniques.
- Discard the solution if it appears cloudy, discolored, or contains particulates.
- Use the reconstituted HGH within the recommended time frame.
- Follow local regulations for disposal of medical waste (e.g., syringes and vials).
By following these steps, you can ensure the HGH is properly mixed and safe for use.
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